Subtype and sequence analysis of the gag genes for HIV-1 strains isolated in Hubei province

VernacularTitle:湖北省HIV-1流行株gag基因序列测定及亚型分析
Author: Xiao-Gang CHU ¹ ; Fa-Xian ZHAN ; Guo-Ping PENG ; Hui-Ping CHEN ; Ting-Hai PENG ; Heng TANG ; Yan LI
Author Information

1. 430060,武汉大学人民医院检验科
Keywords: HIV-1; Genes; Sequence analysis
From: Chinese Journal of Experimental and Clinical Virology 2012;26(6):460-463
CountryChina
Language:Chinese
Abstract: Objective To investigate the characteristic of subtypes and genetic diversity of HIV-1 circulating in Hubei province and its molecular epidemiological linkages with regard to risk factors of viral transmission.Methods plasma samples of 80 diagnosed individuals was characterized.The gene fragments of gag were amplified by reverse transcriptase polymerase chain reaction (RT-PCR) and HIV-1 genotypes were determined based on the nucleotide sequences of gag region.Results Seven HIV-1 group M subtypes or CRF including B,B',G,CRF01-AE,CRF07-BC,CRF08-BC and CRF15-01B were identified.CRF01-AE was found to be the most dominant subtype (48.4％) followed by CRF7-BC (22.6％) and B' (12.9％).Conclusion The data from this study indicate the existence of multiple HIV-1 subtypes or CRFs in Hubei province and the surveillance of HIV-1 gene variation should be paid more attention to.