Flavonoid glycosides from callus cultures of Dysosma versipellis.
- Author:
Ri-Dao CHEN
1
;
Rui-Gang DUAN
1
;
Jian-Hua ZOU
1
;
Jun-Wei LI
1
;
Xiao-Yue LIU
2
;
Hai-Yan WANG
2
;
Qiu-Hong LI
3
;
Jun-Gui DAI
1
Author Information
- Publication Type:Journal Article
- Keywords: Dysosma versipellis; callus cultures; chemical constituent; flavonoid glycoside
- From: China Journal of Chinese Materia Medica 2016;41(1):87-91
- CountryChina
- Language:Chinese
- Abstract: Various chromatographic techniques, including silica gel column chromatography, Sephadex LH-20, preparative thin-layer chromatography, and preparative HPLC, were employed to isolate the chemical constituents from callus cultures of Dysosma versipellis. Structures of the compounds were elucidated based on UV, IR, MS and NMR spectroscopic data analysis. Totally, seven flavonoid glycosides were isolated from the 95% ethanol extract of the callus cultures and identified as kaempferol-3-O-[6″-(3″'-methoxy)-malonyl]-β-D-glucopyranoside(1), kaempferol-3-O-(6″-O-acetyl)-β-D-glucopyranoside(2), kaempferide-3-O-β-D-glucopyranoside(3), kaempferol-3-O-β-D-glucopyranoside(4), isoquercitrin(5), quercetin-4'-O-β-D-glucopyranoside(6) and kaempferol-3-(6″-malonyl)-β-D-glucopyranoside(7), respectively.All these compounds were isolated from callus cultures of D. versipellis for the first time.Compounds 1, 2, 3, 6 and 7 were firstly obtained from plant materials of D. versipellis, and compound 1 was a new compound.
