Cloning, expression and antiviral activity of arctic fox (Alopex lagopus) interferon-gamma gene.
- Author:
Hailing ZHANG
1
;
Xiuli CHAI
;
Guoliang LUO
;
Fengxue WANG
;
Li YI
;
Xiqun SHAO
;
Xijun YAN
Author Information
1. Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antiviral Agents;
pharmacology;
Base Sequence;
Cloning, Molecular;
Escherichia coli;
genetics;
metabolism;
Foxes;
genetics;
Interferon-gamma;
genetics;
pharmacology;
Molecular Sequence Data;
Recombinant Fusion Proteins;
genetics;
pharmacology
- From:
Chinese Journal of Biotechnology
2008;24(9):1625-1630
- CountryChina
- Language:Chinese
-
Abstract:
In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma(VuIFN-gamma), We have isolated the cDNA encoding arctic fox (Alopex lagopus) VuIFN-gamma. This cDNA encodes a 23 amino acid signal peptide and a 144 amino acid mature protein, which shares 99.8% or 99.4% for nucleotide identity with silver fox and canine, respectively, and 100% for amino acid identity. Expression of recombinant mature arctic fox interferon gamma (mVuIFN-gamma) in bacterial system was confirmed by SDS-PAGE and Western blotting analysis. Recombinant VuIFN-gamma showed higher antiviral activity against vesicular stomatitis virus in cultured Vero and MDCK by inhibiting virus induced cytopathic effect, In view of the immunomodulatory and antiviral activities of VuIFN-gamma, it may provide a basis for further research on antiviral therapy of recombinant VuIFN-gamma in economic animal practice.
