Adenovirus mediated IL-24 gene expression inhibits growth of human glioma cell in vitro.
- Author:
Yunbo SHAN
1
;
Weihua SHENG
;
Yufeng XIE
;
Tielian LIU
;
Yingying JING
;
Zhiqing HU
;
Jicheng YANG
Author Information
1. Department of Celluar and Molecular Biology, Medical School, Suzhou University, Suzhou 215123, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Apoptosis;
Brain Neoplasms;
genetics;
pathology;
Cell Proliferation;
drug effects;
Genetic Therapy;
Glioma;
genetics;
pathology;
Humans;
Interleukins;
genetics;
metabolism;
Recombination, Genetic;
Tumor Cells, Cultured
- From:
Chinese Journal of Biotechnology
2009;25(2):279-286
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the inhibitory effect and anti-cancer mechanism of adenovirus mediated IL-24 gene expression on the human U251 glioma cell. U251 glioma cells were infected with Ad-IL-24 at various multiplicity of infection (MOIs). Cell proliferation was determined by MTT assay. Cell apoptosis was detected by flow cytometry and Hochest staining. The transcription of apoptosis-related genes was analyzed by reverse transcription-PCR (RT-PCR), and the expression of Cleaved Caspase-3 was analyzed by Western blotting. The result showed that the growth of U251 glioma cells was significantly inhibited by Ad-IL-24 at the MOI of 100. The apoptotic rate of U251 glioma cells was 42% 72 h after infection with Ad-IL-24. Four days after infection, the growth of the U251 glioma cells was inhibited to 50%. RT-PCR showed that Ad-IL-24 not only up-regulated expression of bax/bcl-2, ICE, C-myc, p53 and down-regulated the expression of HIF-1alpha, but also enhanced Caspase-3 activation, eventually resulting apoptosis. Taken together, these results suggest that infection of U251 glioma cells with Ad-IL-24 can inhibit growth and induce apoptosis significantly by the regulation of apoptosis-related genes.
