Establishment and identification of a H-2 completely mismatched microtransplantation model of leukemia mouse.
10.7534/j.issn.1009-2137.2014.03.038
- Author:
Hui-Hui WU
1
;
Tie-Qiang LIU
1
;
Xue-Dong SUN
1
;
Xiao-Mei HUANG
1
;
Rui ZHANG
1
;
Zhi-Qiang LIU
1
;
Qiu-Hong MAN
1
;
Ya-Jing HUANG
1
;
Qi-Yun SUN
1
;
Hong-Li ZUO
1
;
Jian-Hui QIAO
1
;
Chang-Lin YU
1
;
Kai-Xun HU
1
;
Hui-Sheng AI
1
;
Mei GUO
2
Author Information
1. Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
2. Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China. E-mail: guom196801@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Disease Models, Animal;
Female;
Hematopoietic Stem Cell Transplantation;
methods;
Leukemia;
therapy;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Transplantation Chimera;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2014;22(3):779-784
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to establish and identify a H-2 completely mismatched microtransplantation model of leukemia mouse. The recipients were female BALB/c mice, while donors were male C57BL/6J mice. Recipients were inoculated intravenously with 1×10(6) of WEHI-3 cells, a cell line of myelomonocytic leukemia. Donors received 100 µg/kg G-CSF mobilization through hypodermic injection, every 12 hours, and it last 5 days. Chemotherapy regimens was MA (mitoxantrone+cytarabine), and it last 4 days. Recipients were given chemotherapy conditioning without GVHD prophylaxis after inoculation of leukemic cells for 2 days, and within 8 hours after last chemotherapy received donor mobilized spleen mononuclear cells (sMNC). The number of sMNC was (3, 6, 12) ×10(7), respectively. The early death rate, recovery level of WBC in peripheral blood and leukemia load were compared between chemotherapy and microtransplantation groups. The donor chimerism was detected by RT-PCR. From the clinical manifestation and pathological features, the GVHD in recipients was evaluated. The results showed that the early mortality in chemotherapy group was 25%, meanwhile those in the (3, 6, 12)×10(7) groups were 16.67%, 8.33%, 8.33%, respectively. The(3, 6)×10(7) groups has a stronger hematopoietic recovery capability than that in chemotherapy and 12×10(7) groups (P < 0.05) . There were more leukemic cells in chemotherapy mice than that in microtransplantation mice (P < 0.01) , and (12, 6)×10(7) groups had lower leukemia load than that in 3×10(7) group (P < 0.05) . No signs of GVHD were observed in microtransplantation mice. The donor microchimerism could be discovered at eraly 2 weeks after donor cell transfusion. It is concluded that a H-2 completely mismatched microtransplantation model of leukemia mouse has been successfully established, and it will provide a experimental base for studying microtransplantation in clinic.
