Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis.
- Author:
Xiu-Juan QU
1
;
Ming-Fang ZHAO
;
Yun-Peng LIU
;
Yue-E TENG
;
Jing-Lei QU
;
Ye ZHANG
;
Ling XU
;
Ying-Chun LI
;
Ke-Zuo HOU
Author Information
1. Department of Medical Oncology, The First Clinical College, China Medical University, Shenyang 110001, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Bufanolides;
pharmacology;
Cell Proliferation;
drug effects;
Down-Regulation;
Gene Expression Regulation, Leukemic;
HL-60 Cells;
Humans;
Intracellular Signaling Peptides and Proteins;
metabolism;
Protein-Tyrosine Kinases;
metabolism;
Proto-Oncogene Proteins c-cbl;
metabolism;
Signal Transduction;
Syk Kinase
- From:
Journal of Experimental Hematology
2009;17(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis. Cell viability was tested by trypan blue staining and apoptosis was detected by using flow cytometry. The expressions of CBL and CBL-b and the phosphorylation of SYK were detected by using immunoprecipitation and Western blot. The results showed that Bufalin inhibited HL-60 cell proliferation in time- and dose-dependent manners. IC(50) of suppressing cell viability at 24, 48 and 72 hours were about 26.3, 7.8 and 2.0 nmol/L respectively. The high dose of bufalin already induced apoptosis of HL-60 cells at 8 hours. SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin. It is concluded that SYK activation and CBL protein down-regulation may be involved in Bufalin-induced HL-60 cell apoptosis.
