Determination of equilibrium solubility and n-octanol/water partition coefficient of pulchinenosiden D by HPLC.
- Author:
Xiao-Yong RAO
;
Shan YIN
;
Guo-Song ZHANG
;
Xiao-Jian LUO
;
Hui JIAN
;
Yu-Lin FENG
;
Shi-Lin YANG
- Publication Type:Journal Article
- MeSH: 1-Octanol; chemistry; Acetonitriles; chemistry; Biological Availability; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; pharmacokinetics; Gastrointestinal Tract; metabolism; Humans; Hydrogen-Ion Concentration; Intestinal Absorption; Kinetics; Methanol; chemistry; Pulsatilla; chemistry; Solubility; Solvents; chemistry; Water; chemistry
- From: China Journal of Chinese Materia Medica 2014;39(9):1593-1596
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the equilibrium solubility of pulchinenosiden D in different solvents and its n-octanol/water partition coefficients.
METHODCombining shaking flask method and high performance liquid chromatography (HPLC) to detect the n-octanol/water partition coefficients of pulchinenosiden D, the equilibrium solubility of pulchinenosiden D in six organic solvents and different pH buffer solution were determined by HPLC analysis.
RESULTn-Octanol/water partition coefficients of pulchinenosiden D in different pH were greater than zero, the equilibrium solubility of pulchinenosiden D was increased with increase the pH of the buffer solution. The maximum equilibrium solubility of pulchinenosiden D was 255.89 g x L(-1) in methanol, and minimum equilibrium solubility of pulchinenosiden D was 0.20 g x L(-1) in acetonitrile.
CONCLUSIONUnder gastrointestinal physiological conditions, pulchinenosiden D exists in molecular state and it has good absorption but poor water-solubility, so increasing the dissolution rate of pulchinenosiden D may enhance its bioavailability.