Quantitative determination of 5 active ingredients in different harvest periods of Ligusticum chuanxiong by HPLC.
- Author:
Jin-Liang LIU
;
Qiao-Jia FAN
;
Shun-Lin ZHENG
;
Jie TAN
;
Juan ZHOU
;
Ji-Chao YUAN
;
Shi-Min YANG
;
Fan-Lei KONG
- Publication Type:Journal Article
- MeSH:
4-Butyrolactone;
analogs & derivatives;
analysis;
Acetic Acid;
chemistry;
Acetonitriles;
chemistry;
Benzofurans;
analysis;
Chromatography, High Pressure Liquid;
methods;
Coumaric Acids;
analysis;
Ligusticum;
chemistry;
Methanol;
chemistry;
Solvents;
chemistry;
Time Factors
- From:
China Journal of Chinese Materia Medica
2014;39(9):1650-1655
- CountryChina
- Language:Chinese
-
Abstract:
A simple and quick method is described for the determination of ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A and ligustilide in rhizomes of Ligusticum chuanxiong. The 5 active ingredients in the sample was extracted using 40% ethanol and analyzed by reversed-phase high performance liquid chromatography (HPLC). Chromatography separation was performed using Agilent 1100 series HPLC system with a Symmetry C18 column and gradient elution with a mixture of three solvents : solvent A, acetonitrile, solvent B, methanol and solvent C, 1% aqueous acetic acid, 0 min to 5 min A: B: C 20: 40: 40, 5 min to 30 min A: B: C 60 to 100 : 0 : 40 to 0. The effluent was monitored using a VWD detector set at 321 nm (0-4.3 min) and 275 nm (4.31-30 min). The flow rate was set at 1 mL x min(-1) and the injection volume was 10 microL. The column temperature was maintained at 35 degrees C. The calibration curve was linear (r > or = 0.99) over the tested ranges. The average recovery was 94.44%-103.1% (n = 6). The method has been successfully applied to the analysis in different harvest periods of L. chuanxiong samples. In this paper, single-factor randomized block design to study the 5 components content of L. chuanxiong on ten collecting stages. For the L. chuanxiong collected from April 15th to May 30rd, the content of 5 ingredients increased primarily, and then decreased. Determine the appropriate harvest time has important significance to the promotion of the quality of L. chuanxiong.
