Synthesis and identification of artificial antigens of paneoniflorin.
- Author:
Hui-Hua QU
;
Yan ZHAO
;
Xin SU
;
Na-Na HE
;
Ye SUN
;
Hui KONG
;
Yan ZHAO
;
Qing-Guo WANG
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies;
analysis;
Antigens;
chemistry;
immunology;
Drugs, Chinese Herbal;
chemistry;
Enzyme-Linked Immunosorbent Assay;
Glucosides;
chemistry;
immunology;
Male;
Mice;
Mice, Inbred BALB C;
Monoterpenes;
chemistry;
immunology;
Serum Albumin, Bovine;
chemistry;
immunology
- From:
China Journal of Chinese Materia Medica
2014;39(11):2043-2046
- CountryChina
- Language:Chinese
-
Abstract:
Oxidation method with sodium iodide was used to synthesize immunogenic antigen (PF-BSA) and coating antigen (PF-OVA) of paeoniflorin. UV spectroscopy showed that paeoniflorin was successfully conjugated with BSA and OVA. After immunized by PF-BSA, the mice can produce anti-paeoniflorin antibodies specifically. The ELISA test results showed the high titer (1:12 800) and specificity (IC50 = 0.791 mg x L(-1)) of the antiserum from mice injected with PF-BSA. Also, the antiserum showed low cross activities against nine traditional Chinese medicine (TCM) of small molecules. These artificial antigens were successfully synthesized and the anti-paeoniflorin antibody well prepared, which provides the experimental basis for the further study of ELISA and its kit.
