Differential expression of osteopontin and bax, bcl-2 by fluoride.
- Author:
Hui XU
1
;
Jing-min ZHANG
;
Xiu-yun ZHANG
;
Guang-sheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; genetics; Apoptosis Regulatory Proteins; genetics; Cells, Cultured; Epithelial Cells; drug effects; metabolism; Gene Expression Profiling; Kidney Tubules; cytology; Male; Osteopontin; genetics; Proto-Oncogene Proteins c-bcl-2; genetics; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Sodium Fluoride; pharmacology; bcl-2-Associated X Protein; genetics
- From: Chinese Journal of Preventive Medicine 2005;39(2):107-110
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the differential expression of bax, bcl-2 and osteopontin by fluoride in the renal tubular cells in vitro.
METHODSThe renal tubular cells were cultured and exposed to sodium fluoride (NaF) in 1, 5, 7.5, 12.5 mg F-/L level. The transcription level of bax, bcl-2 and osteopontin were investigated by reverse transcription - polymerase chain reaction (RT-PCR).
RESULTSThe expression of bax mRNA in 7.5 and 12.5 mgF-/L groups (optical absorption ratio value was 2.37 +/- 0.18 and 2.64 +/- 0.19 respectively) was significantly increased (P < 0.01). On the contrary, the level of bcl-2 obviously decreased (5 mg F-/L group optical absorption ratio value, 0.80 +/- 0.22, P < 0.05; 7.5 mg F-/L group optical absorption ratio value 0.71 +/- 0.22, P < 0.01). The expression mRNA of osteopontin was significantly increased when cells were exposed to fluoride at 7.5 mg F-/L (optical absorption ratio value 2.01 +/- 0.40 P < 0.01), in that group the tubular cell apoptotic trend was obvious.
CONCLUSIONNaF might induce tubular cell apoptosis via activation of bax expression and bcl-2 suppression. Osteopontin might protect the tubule against apoptosis in a lower fluoride level, but the function should be decreased in higher fluoride level.
