Analysis of full-length gene sequence of a rabies vaccine strain CTN-1 for human use in China.
- Author:
Lei-tai SHI
1
;
Yong-xin YU
;
Jing-hua LIU
;
Jian-rong TANG
;
Xiao-hong WU
;
Shou-chun CAO
;
Jia LI
;
Xiao-min QU
;
Guan-mu DONG
Author Information
1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Genome, Viral;
genetics;
Humans;
Molecular Sequence Data;
Phylogeny;
Rabies;
prevention & control;
virology;
Rabies virus;
classification;
genetics;
immunology;
Sequence Alignment;
Sequence Analysis, DNA;
Sequence Homology, Nucleic Acid;
Viral Vaccines;
genetics
- From:
Chinese Journal of Virology
2010;26(3):195-201
- CountryChina
- Language:Chinese
-
Abstract:
CTN-1 is one of the rabies vaccine strains for human use in China, but there has been no report on the full-length gene sequence of CTN-1. In this study, the full-length gene of CTN-1 was amplified by RT-PCR, each PCR product was cloned into T vector and then sequenced, assemblied and compared with other vaccine strains as well as the wild Chinese rabies isolates. The phylogenetic tree of G gene was constructed and the genetic homology was analyzed. The results revealed that CTN-1 was 11 925nt (GenBank accession number: FJ959397)in length and belonged to the genotype I. The full-length nucleotide homologies among CTN-1 and other rabies virus strains were between 81.5%-93.4%, of which the lowest 81.5% was between CTN-1 strain and bat isolate SHBRV, and the highest 93.4% was between CTN-1 and Chinese isolate HN10. The phylogenetic analysis revealed that the majority of Chinese isolates could be grouped into the same clade with the CTN-1 strain, but aG and some vaccine strains from abroad such as Flury, PM, PV, ERA, RC-HL and a few Chinese strains were grouped in another clade. Comparsion of the G protein genes also showed that the homologies among CTN-1 and most of the Chinese isolates were higher than that of the other vaccine strains to those Chinese strains. Therefore, it suggests that the CTN-1 strain is more suitable and rational to be used for the production of rabies inactivated vaccine in China than the others.
