Impaired effect of BHC80 gene knock-down on the cardiac development in zebrafish.
- Author:
Jia-Yun HOU
1
;
Dong-Li SONG
;
Da-Qing JIN
;
Jing-Ying HU
;
Xiang-Dong WANG
Author Information
1. Zhongshan Hospital, Fudan University, Shanghai 200032, China; School of Life Sciences, Fudan University, Shanghai 200433, China; Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai 200032, China. xiangdong.wang@clintransmed.org.
- Publication Type:Journal Article
- MeSH:
Animals;
Embryonic Development;
genetics;
Gene Expression Regulation, Developmental;
Gene Knockdown Techniques;
Heart;
embryology;
Histone Deacetylases;
genetics;
Mice, Knockout;
Oligonucleotides, Antisense;
RNA, Messenger;
Zebrafish;
embryology;
Zebrafish Proteins;
genetics
- From:
Acta Physiologica Sinica
2013;65(5):547-552
- CountryChina
- Language:Chinese
-
Abstract:
The effect of BHC80 (a component of BRAF-HDAC complex) on development was not well studied, because BHC80 gene knock-out mice died in one day after birth. Interestingly, zebrafish embryos can live, even if their important organs like cardiac system has severe dysfunction, as 25%-40% O2 are supplied through their skin. Therefore, a model of BHC80 gene knock-down zebrafish embryos was established to explore the effect of BHC80 on the early embryonic development. BHC80-morpholino antisense oligonucleotides 2 (BHC80-MO2) was designed and injected into zebrafish embryos to interrupt the correct translation of BHC80 mRNA at one or two cells stage, which was proved by RT-PCR analysis. Two control groups, including non-injection group and control-MO (con-MO) injection group, and four different doses of BHC80-MO2 injection groups, including 4 ng, 6 ng, 8 ng and 10 ng per embryo were set up. The embryonic heart phenotype and cardiac function were monitored, analyzed and compared between con-MO and BHC80-MO2 groups by fluorescence microscope in vmhc:gfp transgenic zebrafish which express green fluorescent protein in ventricle. The results showed that BHC80-MO2 microinjection effectively knocked down the BHC80 gene expression, because the BHC80-MO2 group emerged a new 249 bp band which reduced 51 bp compared to 300 bp band of con-MO group in RT-PCR analysis, and the 51 bp was the extron 10. The abnormal embryo rate rose with the increase of BHC80-MO2 dosage. The proper BHC80-MO2 injection dosage was 8 ng per embryo, as minor embryos had abnormal phenotype in 4 ng and 6 ng per embryo groups and most embryos died in 10 ng per embryo group. BHC80-MO2 embryos exhibited abnormal cardiac phenotype, including imbalance of the proportion of heart ventricle to atrium, incomplete D-loop, even tubular heart, slow heart rates and cardiac dysfunction. The results from a model of BHC80 gene knock-down zebrafish embryos show that the abnormal cardiac phenotype and cardiac dysfunction of BHC80-MO2 embryos may be one of the probable reasons for the BHC80 gene knock-out mice death, which would provide a good research model to clarify the mechanism of cardiac development.
