Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells.
- Author:
Jun LU
1
,
2
;
Hui-Yue DONG
;
Ling-Jing LIN
;
Qing-Hua WANG
;
Liang-Hu HUANG
;
Jian-Ming TAN
Author Information
1. Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou General Hospital, Fuzhou 350025, China. hlhtiger@
2. com, tanjm156@gmail.com.
- Publication Type:Journal Article
- MeSH:
Activating Transcription Factor 4;
metabolism;
Apoptosis;
DNA-Binding Proteins;
metabolism;
Endoplasmic Reticulum Stress;
Heat-Shock Proteins;
metabolism;
Humans;
Membrane Glycoproteins;
metabolism;
Mesenchymal Stromal Cells;
cytology;
drug effects;
Palmitates;
pharmacology;
Regulatory Factor X Transcription Factors;
Transcription Factor CHOP;
metabolism;
Transcription Factors;
metabolism;
Umbilical Cord;
cytology;
X-Box Binding Protein 1
- From:
Acta Physiologica Sinica
2013;65(6):600-606
- CountryChina
- Language:Chinese
-
Abstract:
The saturated free fatty acid (FFA), palmitate, could induce apoptosis in various cell types, but little is known about its effects on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). Here, we investigated whether palmitate induced apoptosis and endoplasmic reticulum (ER) stress in hUC-MSCs. hUC-MSCs were stained by labeled antibodies and identified by flow cytometry. After administration with palmitate, apoptotic cell was assessed by flow cytometry using the Annexin V-FITC/7-AAD apoptosis detection kit. Relative spliced XBP1 levels were analyzed using semi-quantitative RT-PCR. The mRNA of BiP, GRP94, ATF4 and CHOP were analyzed by real-time PCR. Relative BiP and CHOP protein were analyzed using Western blot analysis. The results showed that hUC-MSCs were homogeneously positive for MSC markers; palmitate increased apoptosis of hUC-MSCs and activated XBP1 splicing, BiP, GRP94, ATF4 and CHOP transcription. These findings suggest that palmitate induces apoptosis and ER stress in hUC-MSCs.
