Effect of high-mobility group box 1 on the proliferation of primary neural stem cells in vitro.
- Author:
Man LI
1
,
2
;
Yong LUO
;
Yuan LI
;
Lin SUN
Author Information
1. Department of Neurology, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China; Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016 , China; Basic Medicine College of Shanxi Medical University, Taiyuan 030001, China; Department of Orthopedics, Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, China. luoyong1998@
2. com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Proliferation;
Cells, Cultured;
HMGB1 Protein;
pharmacology;
JNK Mitogen-Activated Protein Kinases;
metabolism;
Neural Stem Cells;
cytology;
Phosphorylation;
Rats
- From:
Acta Physiologica Sinica
2014;66(4):469-475
- CountryChina
- Language:Chinese
-
Abstract:
The cell counting kit-8 (CCK-8) proliferation assay and diameter measure of neurospheres were used to investigate the effect of high-mobility group box 1 (HMGB1) on proliferation of primary rat neural stem cells (NSCs) in vitro, and c-Jun N-terminal protein kinase (JNK) potent inhibitor SP600125 was used to study the mechanism. The results demonstrated that HMGB1 significantly increased CCK-8 absorbance values and neurosphere diameters at concentrations of 1 and 10 ng/mL at 48 h and 72 h (P < 0.05), and the other HMGB1 concentration groups (0.01, 0.1, 100 ng/mL) showed no significant difference, compared with control group (P > 0.05). HMGB1 at 10 ng/mL significantly increased the NSCs proliferation accompanied by the rising of phosphorylated JNK levels (P < 0.01), and 10 μmol/L SP600125 prevented these effects in HMGB1-cultured NSCs (P < 0.01). In conclusion, low concentration of HMGB1 (1-10 ng/mL) can increase NSCs proliferation, which may play a role by promoting JNK phosphorylation.
