Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro.
- Author:
Yuan WANG
1
;
Chuner CAI
;
Bailin LI
;
Chengchu LIU
;
Peimin HE
Author Information
1. Shanghai Ocean University, Shanghai 201306, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
radiation effects;
Cell Line, Tumor;
Humans;
Lasers;
Liver Neoplasms;
pathology;
Photochemical Processes;
Photochemotherapy;
methods;
Phycobiliproteins;
isolation & purification;
pharmacology;
Phycoerythrin;
isolation & purification;
pharmacology;
Porphyra;
chemistry
- From:
Chinese Journal of Biotechnology
2009;25(9):1417-1423
- CountryChina
- Language:Chinese
-
Abstract:
We studied the effect of photodynamic therapy with phycobiliproteins on human liver cancer cells in vitro. With 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay), we used two phycobiliproteins, R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) prepared from Porphyra yezoensis, to determine the killing rates and apoptosis rates of human liver cancer cells (SMMC-7721) mediated by laser. When the concentration of R-PE was 120 mg/L, the survival rate of human liver cancer cells was 27% after treated by Argon laser with 100 J/cm2 doses, while the survival rate in the control group (without adding R-PE) was 65%. When the C-PC concentration was 120 mg/L, the survival cell rate was 47% after treated by He-Ne laser with 35 J/cm2 dose, while the survival rate in the control group (without adding C-PC) was 70%. After handled only with these two kinds of phycobiliproteins for 72 h, the growth of cancer cells presented significant inhibition. The maximal inhibition rates reached up to 31% with R-PE (120 mg/L concentration) and 27% with C-PC (250 mg/L concentration) respectively. After irradiated by laser for 8 h, the maximal cell apoptosis rates were 31.54% with R-PE and 32.54% with C-PC, respectively. It indicated that R-PE and C-PC extracted from Porphyra yezoensis could develop to new photosensitizers for cancer photodynamic therapy.
