Neodymium Oxide Induces Cytotoxicity and Activates NF-κB and Caspase-3 in NR8383 Cells.

Author: Li Hua HUANG ¹ ; Hua YANG ¹ ; Xin SU ¹ ; Yan Rong GAO ¹ ; Hai Nan XUE ¹ ; Su Hua WANG ¹
Author Information

1. School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, China.
Publication Type:Journal Article
MeSH: Animals; Caspase 3; metabolism; Cell Line; Macrophages, Alveolar; drug effects; enzymology; NF-kappa B; metabolism; Neodymium; toxicity; Oxides; toxicity; Rats; Toxicity Tests
From: Biomedical and Environmental Sciences 2017;30(1):75-78
CountryChina
Language:English
Abstract: We investigated whether Nd2O3 treatment results in cytotoxicity and other underlying effects in rat NR8383 alveolar macrophages. Cell viability assessed by the MTT assay revealed that Nd2O3 was toxic in a dose-dependent manner, but not in a time-dependent manner. An ELISA analysis indicated that exposure to Nd2O3 caused cell damage and enhanced synthesis and release of inflammatory chemokines. A Western blot analysis showed that protein expression levels of caspase-3, nuclear factor-κB (NF-κB) and its inhibitor IκB increased significantly in response to Nd2O3 treatment. Both NF-κB and caspase-3 signaling were activated, suggesting that both pathways are involved in Nd2O3 cytotoxicity.