Genetic modification of bone marrow mesenchymal stem cells with human CXCR4 gene and migration in vitro.
- Author:
Yue ZHANG
1
;
Lailiang OU
;
Zhaokang CHENG
;
Xiaohua JIA
;
Nianfa GAO
;
Deling KONG
Author Information
1. College of Biotechnology, Tianjin University of Science & Technology, Tianjin Key Laboratory of Industrial Microbiology, Tianjin 300457, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
cytology;
Cell Movement;
Cells, Cultured;
Chemokine CXCL12;
pharmacology;
Genetic Vectors;
genetics;
Humans;
Mesenchymal Stromal Cells;
cytology;
metabolism;
Receptors, CXCR4;
biosynthesis;
genetics;
Retroviridae;
genetics;
metabolism;
Transduction, Genetic;
Transfection
- From:
Journal of Biomedical Engineering
2009;26(3):595-600
- CountryChina
- Language:Chinese
-
Abstract:
This study was amied to construct CXCR4 gene modified bone marrow mesenchymal stem cells (MSCs), and investigate the effect of CXCR4 expression on MSCs migration. The retrovirus vector pMSCV-CXCR4-IRES-GFP that expresses human CXCR4 gene was cloned,the MSCs were transduced by the virus, and the expression of OXCR4 was analyzed by FACS, RT-PCR and immunofluorescence staining. The migration assay was performed using Transwell method in the presence of SDF-1. FACS results showed that 46% of the transduced MSCs were CXCR4 positive, and 57% were GFP positive. The expression of CXCR4 in MSCs was also confirmed by RT-PCR and immunostaining. The migration of MSCs was induced by SDF-1 and was strongly dependent on CXCR4 expression. The concentration of SDF-1 had effect on the migration and the transmigration rate of CXCR4 modified; the amount of MSCs was 5-fold higher than that of untransduced MSCs when the optimal concentration rose to 50 ng/ml. These data indicate that SDF-1/CXCR4 plays an important role in MSCs migration ,and the CXCR4 genetic modification approach could be applied to enhance cell homing, and engraftment in MSCs therapy.
