Up-regulation of Fas is related to apoptosis of HeLa cells induced by co-immobilized TNF-alpha/IFN-gamma.
- Author:
Ruifang SUN
1
;
Zeqin FANG
;
Jianting ZHENG
;
Zherui WU
;
Jinwei QIU
;
Weifang LI
;
Dongqin ZHAO
;
Shujun LIANG
;
Yanqing GUAN
Author Information
1. College of Life Science, South China Normal University, Guangzhou 510631, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
genetics;
Drug Synergism;
HeLa Cells;
Humans;
Immobilized Proteins;
chemistry;
pharmacology;
Interferon-gamma;
chemistry;
pharmacology;
Tumor Necrosis Factor-alpha;
chemistry;
pharmacology;
Up-Regulation;
fas Receptor;
metabolism
- From:
Journal of Biomedical Engineering
2009;26(3):615-619
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
