The effect of co-immobilized TNF-alpha/IFN-gamma on mitochondrial membrane potential of HeLa cells.
- Author:
Lianmin ZHONG
1
;
Wenwen WANG
;
Huimin TAO
;
Yanqing GUAN
Author Information
1. College of Life Science, South China Normal University, Guangzhou 510631, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Dose-Response Relationship, Drug;
HeLa Cells;
Humans;
Immobilized Proteins;
pharmacology;
Interferon-gamma;
pharmacology;
Membrane Potential, Mitochondrial;
drug effects;
Mitochondrial Membranes;
drug effects;
physiology;
Tumor Necrosis Factor-alpha;
pharmacology
- From:
Journal of Biomedical Engineering
2009;26(5):972-977
- CountryChina
- Language:Chinese
-
Abstract:
This study inquired into the mechanisms of co-immobilized cytokines and free cytokines-induced apoptosis on HeLa cells. With the use of photochemical fixed method, TNF-alpha/IFN-gamma were co-immobilized on a 24-well polystyrene culture plate. HeLa cells were stained with fluorescent probe JC-1 to detect the changes of mitochondrial membrane potential (deltapsim), and then were examined by flow cytometry. The results showed that co-immobilized cytokines could induce the apoptosis of HeLa cells in a dose-independent manner. When treated with low-dose of co-immobilized cytokines (20ng/ml), the mitochondrial membrane potential (deltapsim) of HeLa cells continually decreased in 6 days. These indicate that low dose co-immobilized cytokines have a long-term of apoptosis-inducing effect on HeLa cells. We assume that there is close relationship between the mitochondrial membrane potential decrease and the apoptosis of HeLa cells.
