Construction and antigenic evaluation of a recombinant MVA virus-like particle expressing HBV C gene.
- Author:
Xiang-ling LUAN
1
;
Wei KONG
;
Su-jun LIU
;
Li LEI
;
Yan HU
;
Jun HOU
;
Hong-hui SHEN
;
Yi-chen WU
;
Shao-li YOU
;
Pan-yong MAO
;
Shao-jie XIN
Author Information
- Publication Type:Journal Article
- MeSH: Genes, Viral; Genetic Vectors; Hepatitis B Core Antigens; genetics; Recombination, Genetic; Vaccinia virus; genetics
- From: Journal of Southern Medical University 2008;28(2):252-254
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the virus-like parcel expressing hepatitis B virus (HBV) C gene and identify its immunogenicity.
METHODSHBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara (MVA).
RESULTSpSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immunogenicity.
CONCLUSIONThe MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.
