Influence of fusion protein of IBDV VP2 and chicken interleukin-2 on immune response in chicken.
- Author:
Chen WANG
1
;
Zhanqin ZHAO
;
Chunjie ZHANG
;
Yichen LIU
;
Ke DING
;
Yinju LI
;
Xiangchao CHENG
;
Puyan CHEN
Author Information
1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
biosynthesis;
blood;
Chickens;
immunology;
Immunoglobulin G;
blood;
Interleukin-2;
biosynthesis;
genetics;
Pichia;
genetics;
metabolism;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
immunology;
Th1 Cells;
immunology;
Th2 Cells;
immunology;
Vaccines, Subunit;
immunology;
Viral Structural Proteins;
biosynthesis;
genetics;
Viral Vaccines;
immunology
- From:
Chinese Journal of Biotechnology
2010;26(4):476-482
- CountryChina
- Language:Chinese
-
Abstract:
In order to research immunogenicity of the recombinant rVP2-IL-2 fusion protein, we obtained the rVP2-IL-2 fusion protein using Pichia pastoris expression system, and then evaluated its potential to induce immune responses in chicken. The effect was determined in the form of protective anti-IBDV VP2 titers, antibodies (IgG1 and IgG2a), lymphocyte proliferation, the levels of interferon-gamma and interleukin-4 cytokines, and challenge experiment. Antibody titers and proliferation lymphocyte level suggested that the fusion protein could elicit specific humoral immune and cellular immune responses, antibody sub-type results indicated that the rVP2-IL-2 fusion protein induced secretion both of IgG1 and IgG2a. The seem result elicited from cytokines ELISA test, secretion of both of Th1 (gamma-IFN) and Th2 (IL-4) were induced by the rVP2-IL-2 fusion protein. Challenge experiment result shown that chicken immunized the rVP2-IL-2 fusion protein obtained 85% protection. These results confirm that the fusion protein enhances the protection against IBDV through both humoral and cell-mediated immunity, and thus could serve as a candidate for the development of IBDV subunit vaccine.
