Substance P up-regulates the TGF-beta 1 mRNA expression of human dermal fibroblasts in vitro.
- Author:
Dahai HU
1
;
Bi CHEN
;
Xiongxinag ZHU
;
Ke TAO
;
Chaowu TANG
;
Jianbo WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; Cells, Cultured; Dermis; cytology; Fibroblasts; cytology; drug effects; metabolism; Gene Expression; drug effects; Neurokinin-1 Receptor Antagonists; Quinuclidines; pharmacology; RNA, Messenger; Substance P; metabolism; pharmacology; Transforming Growth Factor beta; genetics; Transforming Growth Factor beta1; Up-Regulation
- From: Chinese Journal of Plastic Surgery 2002;18(4):234-236
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of substance P in the formation of hypertrophic scar.
METHODSDermal fibroblasts derived from human normal skin were cultured with substance P alone or together with selective non-peptide NK-1 tachykinin antagonist, L-703, 606 oxalate salt. The effect of substance P on proliferation of fibroblasts was measured by MTT assay. Furthermore, the TGF-beta 1 mRNA expression in the fibroblasts was determined by in situ hybridization and image analysis.
RESULTSSubstance P enhanced fibroblast proliferation dose-dependently, which showed the maximum rate when the concentration of substance P was 25 ng/ml or higher in the culture media. By 48 hours cultured with 25 ng/ml of substance P, the fibroblasts expressed TGF-beta 1 mRNA more significantly than the fibroblasts without substance P. The effects of substance P on both fibroblast proliferation and TGF-beta 1 mRNA expression could be antagonized by L-703, 606 oxalate salt.
CONCLUSIONThe results suggest that substance P may play an important role in phenotype changes of fibroblasts in skin scarring.
