Effects of noncoding RNA NRON gene regulation on human umbilical vein endothelial cells functions
10.3760/cma.j.issn.0253-3758.2013.03.015
- VernacularTitle:活化T细胞核因子非编码基因对人脐静脉内皮细胞调节作用的实验研究
- Author:
Yu-Ye TANG
1
;
Li-Ke WO
;
Hui CHAI
Author Information
1. 浙江中医药大学生命科学学院
- Keywords:
Endothelial cells;
RNA,untranslated;
NFATC transcription factors
- From:
Chinese Journal of Cardiology
2013;41(3):245-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of noncoding repressor of NFAT (NRON) overexpression or silencing on human umbilical vein endothelial cells (HUVECs)functions.Methods Stable HUVECs cell lines with NRON overexpression and short hairpin RNA (shRNA) interference were obtained.HUVECs,the empty vector pBABE-cell line and the empty vector pSuper-cell line served as controls.Cell proliferations of these cell lines were tested using MTS method,tube formation capacity and migration function were also examined.Results MTS experiments evidenced dose-dependent cells proliferations in all cell lines after 48 h culture with fetal bovine serum (HUVECs,r =0.91 ;pBABE empty vectors cell-line,r =0.88 ; NRON overexpression cell-line,r =0.89 ; pSuper empty vectors cell-line,r =0.95 ; shRNA infererence cell-line,r =0.97).Proliferation capacity was lower in NRON overexpressed HUVECs and was higher in NRON silencing HUVECs compared with pBABE empty vectors treated and normal HUVECs (all P ＜ 0.05).Tube formation and migration functions were also reduced in NRON overexpressed HUVECs [(8.33 ±0.12) roots,(1857 ±65) cells] and increased in shRNA infererence of NRON treated HUVECs [(36.00 ±0.51) roots,(6987 ±50) cells] compared with pBABE empty vectors treated HUVECs [(19.67 ± 1.42) roots,(4411 ± 117) cells],pSuper empty vectors treated HUVECs [(17.33 ± 2.93) roots,(3883 ± 109) cells] and normal HUVECs [(23.33 ± 3.01) roots,(5145 ± 72)cells,all P ＜ 0.05].Conclusion NRON overexpression could reduce and NRON silencing could increase proliferation,tube formation and migration capacities of HUVECs.
