Protective effect of isoorientin on alcohol-induced hepatic fibrosis in rats.
- Author:
Yong-Xin CHEN
1
,
2
;
Quan-Fang HUANG
3
;
Xing LIN
4
;
Jin-Bin WEI
4
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Drugs, Chinese Herbal; administration & dosage; Ethanol; adverse effects; Glutathione Peroxidase; blood; Humans; Liver Cirrhosis; chemically induced; drug therapy; enzymology; prevention & control; Luteolin; administration & dosage; Male; Protective Agents; administration & dosage; Rats; Rats, Wistar; Transforming Growth Factor beta1; metabolism
- From: China Journal of Chinese Materia Medica 2013;38(21):3726-3730
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect and mechanism of isoorientin from Gypsophila elegans on alcohol-induced hepatic fibrosis in rats.
METHODninety healthy male Wistar rats were randomly divided into six groups: the normal control group, the model control group, the colchicines group (positive control, 1.0 mg x kg(-1) x d(-1)), the high, middle and low-dose isoorientin groups (20, 50, 100 mg x kg(-1) x d(-1)). The normal control group received normal saline, while other groups received alcohol to cause hepatic fibrosis. After 24-weeks treatment, the alanine aminotransferase (ALT), aspartate aminotransferase (AST), Interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), hyaluronic acid (HA), laminin (LN), type III precollagen (PCIII), hydroxyproline (Hyp), Myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were assayed according to the manufacturer's instructions, the alpha-SMA and TGF-beta1 were detected by western blotting, and the histopathological changes was observed by H&E staining.
RESULTIsoorientin could improve the liver function by decreasing the activity of ALT, AST, IL-6, TNF-alpha, MDA, MPO, HA, LN, PCIII and Hyp (P < 0.05), increasing the activity of SOD and GSH-Px (P < 0.05), and reducing the expression of alpha-SMA and TGF-beta1 (P < 0.05). In addition, the high and middle-dose isoorientin groups showed more remarkable effect
CONCLUSIONIsoorientin from G. elegans can protect hepatic fibrosis induced by alcohol.
