Role of NADPH oxidase in endothelial cell dysfunction induced by endothelial microparticles.

Fei CHENG; Jun TAO; Jian-qiang FENG; Chun-tao YANG; Yan WANG; Yuan-yuan ZHANG; Xiao-lin ZHANG

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Role of NADPH oxidase in endothelial cell dysfunction induced by endothelial microparticles.

Author: Fei CHENG ^{1
,

2} ; Jun TAO ; Jian-qiang FENG ; Chun-tao YANG ; Yan WANG ; Yuan-yuan ZHANG ; Xiao-lin ZHANG
Author Information

1. Department of Hypertension and Vascular Disease, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. mdcf2006@
2. com
Publication Type:Journal Article
MeSH: Adult; Aged; Cell-Derived Microparticles; drug effects; metabolism; Cells, Cultured; Coronary Disease; pathology; Endothelial Cells; cytology; drug effects; physiology; Female; Humans; Male; Middle Aged; NADPH Oxidases; metabolism; Umbilical Veins; cytology
From: Journal of Southern Medical University 2010;30(5):1103-1106
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the correlation between circulating endothelial microparticles (EMPs) and flow-mediated dialation (FMD) in patients with coronary artery disease (CAD), and investigate the role of NADPH oxidase in endothelial cell dysfunction caused by EMPs.
METHODSFifteen patients with CAD and 15 at high risks of CAD were tested for the level of EMPs and FMD and other biochemical indices, and the correlation between the indices were analyzed. EMPs obtained from cultured human umbilical vein endothelial cells (HUVECs) were phenotyped and used to stimulate the HUVECs, whose ROS and NO production was tested.
RESULTSEndothelial dilation function could be damaged by circulating EMPs in CAD patients. Dysfunction of HUVECs caused by 10(5)/ml EMPs could be reversed by pretreatment with 20 micromol/L apocynin, a NADPH oxidase inhibitor.
CONCLUSIONEndothelial dialation function of the endothelial cells can be damaged by circulating EMPs in patients with CAD in association with NADPH oxidase activation.