Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities.
- Author:
Jiejun JIANG
1
,
2
;
Fenqiang YOU
;
Hongyue MA
;
Jing ZHONG
;
Junfeng ZHANG
;
Zhen ZHAN
;
Yuping TANG
;
Jin'ao DUAN
;
Anwei DING
Author Information
- Publication Type:Journal Article
- MeSH: Amphibian Venoms; chemistry; Animals; Antineoplastic Agents; isolation & purification; pharmacology; Anura; Bufanolides; isolation & purification; pharmacology; Cell Line, Tumor; Chromatography, High Pressure Liquid; methods; Neoplasms; drug therapy
- From: China Journal of Chinese Materia Medica 2011;36(2):205-208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the bioaffinity between 8 bufadienolides(Bu) and tumor cells and analyze the correlation between the bioaffinity and the anti-tumor activities of Bu.
METHODMix and cultivate the chloroform extract of Chansu and MGC-803. Measure the content of 8 Bu in supernatant and cells using HPLC and calculate their affinity rate.
RESULTThe coefficient correlation between the decrease of Bu in cell supernatant after affinity and its MGC-803 restrictive activities, and between the cotent percentage of the free Bu in free cells with its MGC-803 restrictive activities, and between the difference between the decrease and the percentage and its MGC-803 restrictive activities is r = 0.82 (P < 0.05), r = -0.04 and r = 0.83 (P < 0.05) respectively.
CONCLUSIONEight Bu have different levels of affinity with MGC-803 which correlate with their anti-tumor activities.
