Role of MAPK signal transduction pathway phosphorylation induced by granulocyte-macrophage-colony stimulating factor in the proliferation of hormone-independent prostate cancer.
- Author:
Zhao-Hui CHEN
1
;
Hua-Fang WANG
;
Wen JU
;
Heng LI
;
Ya-Jun XIAO
;
Fu-Qing ZENG
;
Chuan-Guo XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Granulocyte-Macrophage Colony-Stimulating Factor; pharmacology; Humans; Ki-67 Antigen; biosynthesis; Male; Mitogen-Activated Protein Kinase 1; metabolism; Mitogen-Activated Protein Kinase Kinases; metabolism; physiology; Neoplasms, Hormone-Dependent; metabolism; pathology; physiopathology; Phosphorylation; Prostatic Neoplasms; metabolism; pathology; physiopathology; Signal Transduction; drug effects
- From: National Journal of Andrology 2006;12(10):872-875
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the phosphorylation intensity of MAPK pathway molecular Erk1/2 and the proliferation of prostate cancer cell line PC-3M.
METHODSFlow cytometry and RT-PCR were employed to study the ratio of different cell cycles and phases, respectively, before and after GM-CSF stimulation. Erk1/2 phosphorylation intensity was examined by Western blot simultaneously.
RESULTSThe rate of PC-3M cells at S and G2/M stages and the expression intensity of Ki-67 increased after GM-CSF incubation in a dose-dependent manner. The phosphorylation intensity of Erk1/2 increased remarkably after stimulation with GM-CSF.
CONCLUSIONThe intensification of Erk1/2 phosphorylation is one important molecular mechanism of the proliferation of hormone-independent prostate cancer.
