Detection of antioxidant active compounds in mori ramulus by HPLC-MS-DPPH.
- Author:
Zuofa ZHANG
1
;
Lian'gen SHI
Author Information
- Publication Type:Journal Article
- MeSH: Antioxidants; analysis; chemistry; isolation & purification; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; pharmacology; toxicity; Mass Spectrometry; Medicine, Chinese Traditional; Morus; chemistry; Plant Extracts; analysis; chemistry; isolation & purification; Plant Leaves; chemistry; Reproducibility of Results; Sensitivity and Specificity; Stilbenes; analysis; chemistry; isolation & purification; Time Factors
- From: China Journal of Chinese Materia Medica 2012;37(6):800-802
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study major antioxidant active compounds in Mori Ramulus.
METHODThe combination of on-line HPLC method and liquid chromatography-mass spectrometry was used to identify major antioxidant compounds and their content was determined by HPLC-DAD (detection wavelength of 320 nm).
RESULTOxyresveratrol was proved to be the major antioxidant compound in Mori Ramulus ethanolic extracts. Using the HPLC-DAD quantitative determination, Mori Ramulus oxyresveratrol had a good linear range of 14-1 260 mg x L(-1) (r = 0.999 96). The average recovery was 98.2% with RSD of 1.2%. This method is simple, rapid, accurate and sensitive. There was rich oxyresveratrol in Mori Ramulus and the content was significantly different according to the mulberry varieties.
CONCLUSIONThe on-line HPLC-MS-DPPH method is applicable for the determination of antioxidant compounds in Mori Ramulus.
