Effect of down-regulation of HE4 gene expression on biologic behavior of ovarian cancer cells.
- Author:
Lei ZHOU
1
;
Ran XIAO
2
;
Ying CHEN
2
;
Jing ZHANG
2
;
Ren-quan LU
2
;
Lin GUO
2
;
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cystadenocarcinoma, Serous; metabolism; Down-Regulation; Female; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Humans; Ovarian Neoplasms; genetics; metabolism; pathology; Plasmids; Proteins; genetics; metabolism; RNA Interference; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Recombinant Proteins; genetics; metabolism; Transfection
- From: Chinese Journal of Pathology 2013;42(10):687-690
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of HE4 gene knockdown on the proliferation, adhesion and invasion of the ovarian cancer cells SKOV3.
METHODSThe knockdown of HE4 gene was performed by RNAi technology. The recombinant plasmids (pSUPER-HE4 shDNAs) were constructed and transfected into human ovarian cancer cells SKOV3. HE4 expression was then identified by real-time PCR and Western blot analysis. The invasion and adhesion ability of transduced cells were determined. In addition, cell proliferation and growth were analyzed by colonies formation assay.
RESULTSKnockdown of HE4 was achieved, and further confirmed by real-time PCR and Western blot. The proliferation of HE4-down-regulated cells was not affected, but the invasion ability of the transfected cells was reduced (P < 0.05) and the adhesion ability was also reduced to 27.3%.
CONCLUSIONHE4 expression is down-regulated effectively by the constructed HE4 shDNA, and thus knockdown of HE4 inhibits the adhesion and invasion of SKOV3 cells.
