ALK fusion gene assessment by fully automatic immunohistochemistry in non-small cell lung cancer.

Lei GUO; Xiuyun LIU; Tian QIU; Yun LING; Ling SHAN; Yongqiang XIE

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ALK fusion gene assessment by fully automatic immunohistochemistry in non-small cell lung cancer.

Author: Lei GUO ¹ ; Xiuyun LIU ¹ ; Tian QIU ¹ ; Yun LING ¹ ; Ling SHAN ¹ ; Yongqiang XIE ^{2
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Author Information

1. Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
2. Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China. E-mail: xyq_cn@
3. com.
Publication Type:Journal Article
MeSH: Adenocarcinoma; genetics; metabolism; Adult; Aged; Aged, 80 and over; Carcinoma, Non-Small-Cell Lung; genetics; metabolism; Female; Gene Rearrangement; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; Lung Neoplasms; genetics; metabolism; Male; Middle Aged; Oncogene Proteins, Fusion; genetics; metabolism; Sensitivity and Specificity
From: Chinese Journal of Pathology 2014;43(2):95-98
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo evaluate the sensitivity and specificity of fully automated immunohistochemistry (IHC), with comparison to FISH, in the detection of EML4-ALK rearrangement in lung adenocarcinoma (ADC); and the use of IHC as a pre-screening tool.
METHODSA total of 404 paraffin-embedded NSCLC samples from surgical resections were tested by IHC with Ventana anti-ALK rabbit monoclonal antibody (D5F3) and ultrasensitive detection kit. ALK rearrangement was further confirmed by FISH.
RESULTSTwenty-nine of 404 lung ADCs (7.2%) were positive for ALK by IHC. ALK positive tumor cells demonstrated strong and diffused granular cytoplasmic staining. All the ALK IHC-positive cases were confirmed to harbor ALK rearrangement by FISH. None of the ALK IHC-negative cases was FISH-positive.
CONCLUSIONSIHC can effectively detect ALK rearrangement in lung cancer. It may provide a reliable and cost-effective diagnostic approach in routine pathologic laboratories for the identification of suitable candidates for ALK-targeted therapy.