Effect of puerarin on PI3K/AKT pathway-mediated apoptosis of PASMCs.
- Author:
Xiao-dan ZHANG
;
Yan-nan YANG
;
Shu-jing WANG
;
Da-ling ZHU
;
Li-wei WANG
;
Jie-jing SHENG
;
Sha-sha SONG
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Cells, Cultured;
Chromones;
pharmacology;
Isoflavones;
pharmacology;
Morpholines;
pharmacology;
Myocytes, Smooth Muscle;
drug effects;
Phosphatidylinositol 3-Kinases;
physiology;
Proto-Oncogene Proteins c-akt;
physiology;
Pulmonary Artery;
cytology;
drug effects;
Rats;
Rats, Wistar;
Signal Transduction;
drug effects
- From:
China Journal of Chinese Materia Medica
2015;40(15):3041-3046
- CountryChina
- Language:Chinese
-
Abstract:
To discuss the effect of puerarin (Pue) on the proliferation of hypoxia-induced pulmonary artery smooth muscle cells (PASMCs) and discuss whether the extracellular signal PI3K/AKT pathway was involved in the Pue-induced PASMC apoptosis. With the serum starvation group (SD group) as the control group, the MTT colorimetry method, Annexin V-FITC apoptosis detection kit and Western blot were used to detect Pue's effect on apoptosis of rat PASMCs. The protein immunoblot assay was used to detect whether PI3K/AKT pathway was involved in the inhibition of hypoxia-induced PASMC apoptosis process. The results show that under normoxic conditions, Pue had no effect on PASMC apoptosis; Under hypoxia conditions, Pue can inhibit PASMC apoptosis; Under normoxic and hypoxic conditions, Pue had no effect on TNF-α expression. Pue can reverse hypoxia-induced Bcl-2 (P <0.01), up-regulate it and down-regulated Bax (P <0.01). Under normoxic conditions, Pue had no effect on P-AKT expression. Both LY294002 and Pue can inhibit hypoxia-induced Bcl-2, up-regulation of P-AKT expression and down-regulation of Bax expression. Compared with the hypoxia + Pue group or the hypoxia + LY294002 group, the hypoxia + Pue + LY294002 group showed more significantly changes in Bcl-2, Bax, P-AKT expressions. The results show that, Pue can inhibit the hypoxic-induced PASMC apoptosis, which may be regulated through PI3K/AKT pathway.
