Preliminary study on effect of Rhodiolae Crenulatae Radix et Rhizoma cell wall-broken decoction pieces on intestinal flora of mice.
- Author:
Ze-rui YANG
;
Gui-mei ZENG
;
Li-hua PENG
;
Miao-miao ZHANG
;
Jin-le CHENG
;
Ruo-ting ZHAN
- Publication Type:Journal Article
- MeSH:
Animals;
Bifidobacterium;
drug effects;
genetics;
growth & development;
Cell Wall;
Denaturing Gradient Gel Electrophoresis;
Intestines;
microbiology;
Lactobacillus;
drug effects;
genetics;
growth & development;
Mice;
Mice, Inbred C57BL;
Polymerase Chain Reaction;
Rhizome;
Rhodiola
- From:
China Journal of Chinese Materia Medica
2015;40(15):3053-3058
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to analyze and compare the effect of cell wall-broken decoction pieces, conventional decoction pieces and conventional powder of Rhodiolae Crenulatae Radix et Rhizoma on the intestinal flora of normal mice. The conventional bacterial culture and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) were adopted for the mice after the oral administration for 14 days. According to the bacterial culture results, the 1/8 dose cell wall-broken decoction pieces group showed fewer Enterococcus and Escherichia coli bacillus but more Lactobacillus and Bifidobacterium than the conventional decoction pieces group and the traditional powder group (P <0.05). Meanwhile, on the basis of the PCR-DGGE results, the 1/8 dose cell wall-broken decoction pieces group revealed the highest Shannon-Wiener index (H) and species richness (S) among the seven groups, with extremely significant differences compared with the normal group (P <0.01), significant differences compared with the conventional decoction pieces group and the conventional powder group (P <0.05) and a high intra-group similarity. In conclusion, the long-term intake of 1/8 dose Rhodiolae Crenulatae Radix et Rhizoma cell wall-broken decoction pieces showed a certain effect in regulating intestinal tract by promoting the growth of Lactobacillus and Bifidobacterium. Furthermore, the intestinal flora community will become more stable.
