IL-1β promotes the hematopoietic support of human umbilical cord mesenchymal stem cells.
10.7534/j.issn.1009-2137.2013.04.037
- Author:
Yue-Ru JI
1
;
Zhou-Xin YANG
;
Li-Na LI
;
Zhi-Bo HAN
;
Ying CHI
;
Zhong-Chao HAN
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cells, Cultured;
Culture Media;
Granulocyte Colony-Stimulating Factor;
secretion;
Granulocyte-Macrophage Colony-Stimulating Factor;
secretion;
Hematopoietic System;
drug effects;
Humans;
Interleukin-1beta;
pharmacology;
Interleukin-6;
secretion;
Mesenchymal Stromal Cells;
cytology;
drug effects;
secretion;
Umbilical Cord;
cytology
- From:
Journal of Experimental Hematology
2013;21(4):1005-1009
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of IL-1β on hematopoietic support of human umbilical cord mesenchymal stem cells (hUC-MSC). 2×10(6) hUC-MSC were seeded in 75 cm(2) flasks, after adherence to wall for 2 h, 10 ng/ml IL-1β was added in hUC-MSC supernatant and cultured for 36 h, then the culture supernatants and cells were harvested. The effect of conditioned medium with/without IL-1β on CD34(+) cell hematopoietic support was observed, mRNA expression changes of hUC-MSC cultured in medium with/without IL-1β were monitored by real time PCR, the differences in hematopoiesis-related factors were detected by ELISA. The results showed that the conditioned culture medium of hUC-MSC with IL-1β enhanced the ability to form colony of CD34(+) cells, especially CFU-G and CFU-GM in vitro; IL-1β promoted the mRNA expression of GM-CSF, G-CSF, IL-6 on MSC; IL-1β also promoted the secretion of GM-CSF, G-CSF, and IL-6 protein from hUC-MSC. It is concluded that IL-1β enhances hematopoietic support capacity especially, capability of MSC to myeloid differentiation.
