Effective of ginkgolides on expression of apoptosis related gene during PC12 cells glucose deprivation.
- Author:
Li-Xin SUN
1
;
Ming YAN
;
Zhen-Zhou JIANG
;
Min CHEN
;
Lu-Yong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; genetics; Apoptosis Regulatory Proteins; genetics; Cell Proliferation; drug effects; Fluorescence; Gene Expression Regulation; drug effects; Ginkgolides; pharmacology; Glucose; deficiency; pharmacology; PC12 Cells; Proto-Oncogene Proteins c-bcl-2; genetics; Proto-Oncogene Proteins c-myc; genetics; RNA, Messenger; biosynthesis; genetics; Rats; Reverse Transcriptase Polymerase Chain Reaction; methods; bcl-2-Associated X Protein; genetics
- From: China Journal of Chinese Materia Medica 2007;32(6):532-535
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effects of ginkgolides on glucose deprivation-induced apoptosis in PC12 cells and the mechanism underlying the protective effect.
METHODPC12 cells were treated under glucose deprivation, and the proliferation was determined by tetrazolium (MTT) assay. Furthermore, the mRNA levels of Bcl-2, Bax, c-myc were measured by Fluorescence Quantitative PCR (FQ-PCR).
RESULTGinkgolides could markedly inhibit the injury of glucose deprivation on the PC12 cells and increase the cell proliferation compared with the model groups (P <0.01). Ginkgolides could up-regulate Bcl-2 and down-regulate Bax and c-myc at 12 h, respectively. There were no significant differences in the Bcl-2 and Bax levels in both groups at 24 h, and ginkgolides only reduced the elevation of c-myc from 4. 32-fold to 2. 87-fold at this time.
CONCLUSIONGinkgolides are able to protect the injured PC12 cells against cell apoptosis. During the early period of glucose deprivation, Bcl-2, Bax and c-myc were regulated to inhibit cell apoptosis by ginkgolides. After that, ginkgolides seems inhibit the apoptosis through attenuating the elevation of c-myc.
