RHD 1227A allele frequency among Rh negative population and random population.
- Author:
Jun-Jie WU
1
;
Xiao-Zhen HONG
;
Xian-Guo XU
;
Kai-Rong MA
;
Fa-Ming ZHU
;
Li-Xing YAN
Author Information
1. Institute of Transfusion Medicine, Blood Center of Zhejiang Province; Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Asian Continental Ancestry Group;
genetics;
Base Sequence;
China;
Chromosome Deletion;
Cloning, Molecular;
Gene Deletion;
Gene Frequency;
genetics;
Humans;
Molecular Sequence Data;
Polymorphism, Genetic;
Rh-Hr Blood-Group System;
genetics;
immunology;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2006;14(6):1234-1237
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the frequency of RHD 1227A allele in Rh negative population and random population, an AS-PCR (allele specific-polymerase chain reaction) method was employed to detect RHD 1227A allele. RHD gene copy was determined by D zygosity test and RHD exon 9 nucleotide sequence analysis. The results showed that among 143 Rh negative donors, forty-one RHD 1227A allele carriers were detected, and 8 (19.51%) out of which were RhCCdee, 32 (78.05%) were RhCcdee, and 1 (2.44%) was RhCcdEe. Thirty-five Rh negative RHD 1227A carriers had RHD gene deletion, and the remaining carriers were RHD 1227A homozygous. Seven (1.43%) individuals were detected with RHD 1227A allele among 489 random donors. They were all G/A heterozygous at RHD 1227 site. Serological test indicated that they were normal Rh positive phenotype. It is concluded that the frequency of RHD 1227A allele is 16.43% among Rh negative population and 0.72% among the random population.
