Determination of calycosin-7-O-beta-D-glucopyranoside in radix astragali by HPLC.
- Author:
Chun-xia LUO
1
;
Ping-chuan LIN
;
Li-hua GU
;
Tao WU
;
Da-zheng WU
;
Zheng-tao WANG
;
Zhi-bi HU
Author Information
- Publication Type:Journal Article
- MeSH: Astragalus membranaceus; chemistry; classification; China; Chromatography, High Pressure Liquid; Ecosystem; Glucosides; analysis; Isoflavones; analysis; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Species Specificity
- From: China Journal of Chinese Materia Medica 2003;28(7):603-606
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETO establish a RP-HPLC method for the determination of calycosin-7-O-beta-D-glucopyranoside in Radix Astragali, and to analyse the calycosin-7-O-beta-D-glucopyranoside content of ten samples of Radix Astragali, collected from different regions.
METHODA Polaris C18(250 mm x 4.6 mm, 5 microns) column was used and a mixture of methanol-water (30:70) was used as the mobile phase at a flow rate of 1.0 mL.min-1. The column temperature was 25 degrees C and the UV detection wavelength was 254 nm.
RESULTThe calibration curve was in good linearity over the range of 0.0106-2.12 micrograms with the regression equation Y = 3035. 97 X - 14.85(r = 0.9999). The average recovery was 95.8% (n = 5, RSD = 1.3%).
CONCLUSIONThe method is simple, quick, sensitive and reproducible. In all of the samples, the calycosin-7-O-beta-D-glucopyranoside contents differ markedly.
