Expression of HOXB4 in cord blood progenitor cells expanded in vitro.
- Author:
Yu-Hong TANG
1
;
Xiao-Ming FEI
;
Wen-Yi SHEN
;
Kou-Rong MIAO
;
Yu-Gui CUI
;
Cheng-Ya WANG
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Bone Marrow Cells;
cytology;
Cell Proliferation;
Cells, Cultured;
Coculture Techniques;
Fetal Blood;
cytology;
Homeodomain Proteins;
biosynthesis;
genetics;
Humans;
Mesenchymal Stromal Cells;
cytology;
Transcription Factors;
biosynthesis;
genetics
- From:
Journal of Experimental Hematology
2006;14(1):89-93
- CountryChina
- Language:Chinese
-
Abstract:
HOXB4, a member of homeobox gene family, is closely related to the self-renewing and proliferative ability of primitive hematopoietic stem/progenitor cells (PHSC/PHPC). This study was aimed to investigate the self-renewing level of cord blood progenitor cells (CBPC) expanded in vitro. The HOXB4 expression at mRNA level was assayed by using real time RT-PCR. The results indicated that as culture prolonged, the total cells, CD34(+) cells greatly increased, however the HOXB4 expression gradually declined, even down to undetectable level similar to that of mature lymphocytes. Meanwhile, it was shown that CD34(+) cells co-cultured with bone marrow mesenchymal stem cells (BM-MSC) could abate the decline of HOXB4 expression. It is concluded that the self-renewing potential of CD34(+) cells gradually decreased during expansion in vitro, co-culture with BM-MSC was helpful to CD34(+) cell expansion and slowed the loss trend of its self-renewal.
