Differentiation of bone marrow mesenchymal stem cells co-cultured with endothelial cells under shear stress.
- Author:
Lin ZHANG
1
;
Yuqnan LI
;
Chuansen ZHANG
;
Yan ZHANG
;
Xiangqun YANG
Author Information
1. Department of Anatomy, Institute of Biomedical Engineering, the Second Military Medical University, Shanghai 200433, China.
- Publication Type:Journal Article
- MeSH:
Actins;
metabolism;
Animals;
Bone Marrow Cells;
cytology;
Calcium-Binding Proteins;
metabolism;
Cell Differentiation;
Cells, Cultured;
Coculture Techniques;
Endothelial Cells;
cytology;
Male;
Mesenchymal Stromal Cells;
cytology;
Microfilament Proteins;
metabolism;
Muscle, Smooth;
cytology;
Myosin Heavy Chains;
metabolism;
Rats;
Rats, Sprague-Dawley;
Shear Strength;
Stress, Mechanical
- From:
Journal of Biomedical Engineering
2009;26(1):85-88
- CountryChina
- Language:Chinese
-
Abstract:
Differentiation of bone marrow mesenchymal stem cells (BMSCs) co-cultured with endothelial cells (ECs) under shear stress was studied. BMSCs and ECs were co-cultured on the two sides of PET membrane, and 20 dyn/cm2 shear stress produced by parallel plate flow chamber was performed after 72 hours. Cell morphology was observed under phase-difference microscope, and the expressions of smooth muscle-alpha-actin (SM-alpha-actin), calponin and smooth muscle myosin heavy chain (SMMHC) of BMSCs were detected by fluorescence immunocytochemistry. The co-cultured BMSCs became smooth muscle-like cells gradually; after 24 hours, the BMSCs started to express SM-alpha-actin. After 48 hours, they expressed SM-alpha-actin and calponin obviously. After 72 hours, obvious expressions of SM-alpha-actin and calponin, but not of SMMHC, were detected. Further static co-culture had no effect on SM-alpha-actin, calponin and SMMH expression of BMSCs; after 24 hours, shear stress induced feeble expression of SM-alpha-actin and obvious expression of SMMHC in co-cultured BMSCs, but it had no effect on the expression of calponin. The results suggest that shear stress may potentiate the differentiation of BMSCs (co-cultured with ECs) into mature smooth muscle-like cells.
