Influence of DNAzymes against cyclin D1 in tumor cell cycle.
- Author:
Dan LI
1
;
Junyi ZHU
;
Yayan ZHOU
;
Xuanming LIU
Author Information
1. Institute of Life Science and Biotechnology, Hunan University, Changsha 410082, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cell Cycle;
drug effects;
Cyclin D1;
antagonists & inhibitors;
genetics;
metabolism;
DNA, Catalytic;
genetics;
metabolism;
pharmacology;
HeLa Cells;
Humans;
Molecular Sequence Data;
RNA, Messenger;
genetics;
metabolism;
Transfection
- From:
Journal of Biomedical Engineering
2009;26(2):374-384
- CountryChina
- Language:Chinese
-
Abstract:
In this study, DNAzymes against cyclin D1 (cyclin D1-DRz) were designed according to the secondary structure of cyclin D1 mRNA which was computed with RNAdraw and Mfold. Cyclin D1-DRz were transfected into tumor cell line u251 and HeLa by oligofectamine. The expression of cyclin D1 was detected by RT-PCR. It was shown that the expression of cyclin D1 gene was suppressed obviously, and the expressions of other cell-cycle related genes such as cyclin E1, cyclin A1 and cyclin B1 were also declined. The cell cycle analysis of tumor cells tansfected with cyclin D1-DRz revealed an arrestment in the G0/G1 phase. In conclusion, the approach is effective and feasible for designing DNAzyme. Cyclin D1-DRz is useful for interfering with the cell cycle procession of tumor cells.
