Hydrocamptothecin promotes the mRNA expression of Wnt signaling inhibitor DKK-1.
- Author:
Lei LA
1
,
2
;
Wu BAN
;
Jin-jun RAO
;
Shu-guang WU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Camptothecin; analogs & derivatives; pharmacology; Cell Line, Tumor; Colorectal Neoplasms; genetics; metabolism; pathology; Flow Cytometry; Humans; Intercellular Signaling Peptides and Proteins; biosynthesis; genetics; Liver Neoplasms; genetics; metabolism; pathology; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; drug effects; Wnt Proteins; metabolism
- From: Journal of Southern Medical University 2008;28(4):623-626
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of hydrocamptothecin on the expression of Wnt signaling pathway inhibitor DKK-1 in tumor cells.
METHODSHuman HepG2, Hep3B, LoVo and U251 cells were treated with the antitumor drug Hydrocamptothecin. DKK-1 mRNA expression in the cells was detected with RT-PCR, and beta-catenin expression was measured by fluorescence-activated cell sorting (FACS).
RESULTSDKK mRNA in Hep3B, HepG2, LoVo and U251 cells was significantly increased after hydrocamptothecin treatment for 24 h, and the percentage of beta-catenin-positive cells and fluorescence intensity for beta-catenin expression was lowered in the cells after the treatment.
CONCLUSIONHydrocamptothecin promotes mRNA expression of Wnt signaling pathway inhibitor DKK-1 in Hep3B, HepG2, LoVo and U251 cells.
