The effects of FRNK on expressions of MMP-2 mRNA and TIMP-2 mRNA in hepatic stellate cells.
- Author:
Juan WEI
1
;
Xiao-lan ZHANG
;
Dong-mei YAO
;
Xiao-xia HUO
;
Jian-gang SHEN
;
Zhi-na DUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Collagen Type I; metabolism; Hepatic Stellate Cells; cytology; metabolism; Matrix Metalloproteinase 2; metabolism; Plasmids; Protein-Tyrosine Kinases; genetics; RNA, Messenger; genetics; Rats; Tissue Inhibitor of Metalloproteinase-2; metabolism; Transfection
- From: Chinese Journal of Hepatology 2008;16(10):757-761
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the effects of FAK-related non-kinase (FRNK) on expressions of type I collagen and matrix metalloproteinase-2 (MMP-2) mRNA and tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA in rat hepatic stellate cells (HSC).
METHODSUsing in vitro cell culture technique, FRNK plasmids were transfected into HSC mediated by cationic liposome. Type I collagen synthesis capability in HSC was examined by 3H-Pro incorporation assay. The levels of FRNK in HSC were assayed by Western blot, and the expressions of MMP-2 and TIMP-2 were assayed by RT-PCR on mRNA levels.
RESULTSThe exposure of HSC to FRNK caused the expression of FRNK protein to be up-regulated, and the FRNK protein contents reached the highest point at 48 h after the transfection, P less than 0.05. The expressions of MMP-2 mRNA were up-regulated by FRNK; the expressions of TIMP-2 mRNA were down-regulated by FRNK; the ratios of MMP-2 mRNA/TIMP-2 mRNA were enhanced by FRNK.
CONCLUSIONAfter FRNK was transfected, the capability of type I collagen synthesis in HSC was inhibited, which may be related to the up-regulation of MMP-2 mRNA/TIMP-2 mRNA.
