Purification of superoxide dismutase from blood erythrocyte by nano magnetic chitosan microspheres.
- Author:
Baoquan WANG
;
Juan PING
;
Feng LI
;
Yongzhou ZHANG
;
Cui LIU
;
Xiaobin PANG
- Publication Type:Journal Article
- MeSH:
Chitosan;
chemistry;
Erythrocytes;
enzymology;
Glutaral;
chemistry;
Magnetics;
Microspheres;
Polymers;
Spectroscopy, Fourier Transform Infrared;
Superoxide Dismutase;
isolation & purification
- From:
Chinese Journal of Biotechnology
2014;30(6):990-994
- CountryChina
- Language:Chinese
-
Abstract:
Nano magnetic microspheres prepared by chitosan and poly acylic acid were applied to purifying superoxide dismutase from blood erythrocyte. Chitosan-polyacyilc acid graft copolymer was synthesized by free radical graft copolymerization with potassium persulfate as inititator. To prepare Fe3O4 magnetic fluids with chemical coprecipitation, chitosan-polyacylic nano magnetic microspheres were prepared with glutaraldehyde as crosslinking agent. Structure of nano magnetic microspheres was detected by FT-IR spectrometer. Particle size and morphology were characterized by JEM-4000EX technology. Chitosan-polyacylic nanometer microspheres have good paticle cize distribution, magnetic responsiveness and protein adsoption. Activity, product yield and activity recovery of SOD after purification reached 6 727 U/mg, 21.1%, and 85.7% respectively. Purification of blood superoxide dismutase by chistosan-polyacylic acid microspheres has its renewable and feasible nature.
