Variation of endogenous hormones in formation of microtuber of Dioscorea opposite in vitro.
- Author:
Li CHANG
1
;
Jianping XUE
;
Yunxian SONG
;
Wei SHENG
;
Conghui XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Abscisic Acid; metabolism; Cyclopentanes; metabolism; Dioscorea; growth & development; metabolism; Gibberellins; metabolism; Indoleacetic Acids; metabolism; Isopentenyladenosine; analogs & derivatives; metabolism; Oxylipins; metabolism; Plant Growth Regulators; metabolism; Plant Tubers; growth & development; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(21):2818-2821
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThrough analysis of variation and function of 5 main endogenous hormones in the formation of microtuber of Dioscorea opposite in vitro to explore the physiological and biochemical mechanism of microtuber development.
METHODWhen microtubers were induced on MS + 6-BA 1.5 mg x L(-1) + NAA 1.5 mg x L(-1) + sucrose 5% medium, the endogenous hormones were isolated during different formation stages of microtubers, then purified and detected with enzyme-linked immunosorbent assays (ELISA).
RESULTThe results showed that GA3 slightly decreased in initial period, rose suddenly 20 days later, and than decreased. IAA showed a dropping tendency in the total course, ABA and ZR increased in a long period, dropped at last. JA continuously rose and never dropped, GA3 and ABA and the ratio of GA3 and JA varied obviously.
CONCLUSIONIAA, ABA, JA , ZR and GA3 play an important role in controlling formation of microtubers in D. opposite in vitro.
