Determination of flavones in different origin and parts of Bupleurum smithii var. parvifoliaum by UPLC-PDA.
- Author:
Fangling TANG
1
;
Guangming CAI
;
Bo YUAN
;
Zhuoyong ZHANG
Author Information
1. China Military Institute of Chinese Materia Mdeica, 302 Military Hospital, Beijing 100039, China.
- Publication Type:Journal Article
- MeSH:
Bupleurum;
chemistry;
China;
Chromatography, High Pressure Liquid;
instrumentation;
methods;
Drugs, Chinese Herbal;
analysis;
standards;
Flavones;
analysis;
Quality Control
- From:
China Journal of Chinese Materia Medica
2010;35(21):2874-2876
- CountryChina
- Language:Chinese
-
Abstract:
An ultra performance liquid chromatography method has been developed for determination of flavones in different origin and different parts from Bupleurum smithii var. parvifoliaum. The separation was performed at 30 degrees C on an Acquity UPLC BEH C18 column eluted with methanol and 0.1% phosphoric acid solution as mobile phases in gradient elution. The detection wavelength was set at 256 nm and the flow rate was 0.4 mL x min(-1). There flavones of rutin, quercetin and isorhamnetin were separated well, the linear calibration curves were obtained over the ranges of 0.106-1.06 microg for rutin (r = 0.999 8, n = 6), 0.004 87-0.048 7 microg for quercetin (r = 0. 999 7, n = 6), 0.022 0-0.220 microg for isorhamnetin (r = 0.999 8, n = 6). The mean recoveries of the three compounds were 99.3%, 97.8%, 98.9% with RSD of 2.4%, 3.0%, 3.2%. The result showed that the method is convenient and feasible for determination of the flavone content in B. smithii var. parvifoliaum.
