Preliminary Analysis of Parkinson-like Motor Coordination Abnormityin Brain-specific hS100B Transgenic Mice.
10.3881/j.issn.1000-503X.2017.02.013
- VernacularTitle:脑组织特异表达hS100B转基因小鼠帕金森样运动协调能力改变的初步分析
- Author:
Jialin LIU
1
;
Fang ZHENG
2
;
Yan LONG
2
;
Liu TONG
2
;
Yuan ZHENG
2
;
Xiaoqing LIU
2
;
Chuan QIN
3
Author Information
1. 1 Department of Pathology,Hebei University Medical College,Baoding,Hebei 071000,China.
2. 2 Department of Geratology,the First Central Hospital of Baoding,Baoding,Hebei 071000,China.
3. 3 Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences,Comparative Medicine Center,Peking Medical College,Beijing 100021,China.
- Publication Type:Journal Article
- From:
Acta Academiae Medicinae Sinicae
2017;39(2):240-246
- CountryChina
- Language:English
-
Abstract:
Objective To investigate the role of S100B in the development of Parkinson's disease (PD) and explore the possibility of brain-specific S100B transgenic mice as PD animal model. Methods The hS100B transgenic mice were established. The mice were divided into S100B transgenic group (TG),S100B knockout group (KG),and the non-transgenic control group (CG). Motor coordination ability of mice was measured by the rota-rod and pole-climbing test. The expressions of S100B,dopamine D1 receptor,dopamine D2 receptor,G protein-coupled receptor kinase (GRK)2,GRK5,and tyrosine hydroxylase in brain tissue were detected by reverse transcription-polymerase chain reaction and Western blot. The levels of tyrosine,levodopa,dopamine,and homovanillic acid in brain tissue were measured by high-performance liquid chromatography coupled with fluorescence detection. Results Compared with CG,the S100B protein expression in brain tissue significantly increased in TG (P<0.05);the motor coordination ability of mice showed progressive decline (P<0.05);the mRNA and protein expressions of dopamine D2 receptor and GRK2 significantly decreased (P<0.05);the levels of levodopa,dopamine,and homovanillic acid were significantly elevated (P<0.05);the expression of tyrosine hydroxylase was also down-regulated,although there was no significant difference (P>0.05). Compared with CG,there was no obvious change of the above indicators in KG (all P>0.05). Conclusion S100B plays an important role in the motor coordination abnormity of PD. The brain-specific S100B transgenic mice can be used in research on the role of S100B gene in the development of PD.
