Correlation between expression of apoptosis-related gene pnas-2 and leukemia.
- Author:
Hong-Hui HUANG
1
;
Jian-Yi ZHU
;
Ji-Hua ZHONG
;
Hai-Rong WANG
;
Hua ZHONG
;
Jie-Ying HAN
;
Fang-Yuan CHEN
Author Information
1. Department of Hematology, Renji Hospital, Shanghai Jiaotong University Medical College, Shanghai 200001, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Antineoplastic Combined Chemotherapy Protocols;
therapeutic use;
Apoptosis;
drug effects;
Apoptosis Regulatory Proteins;
metabolism;
Arsenicals;
pharmacology;
Female;
Humans;
K562 Cells;
Leukemia;
pathology;
Leukemia, Myeloid, Acute;
drug therapy;
genetics;
pathology;
Male;
Middle Aged;
Sulfides;
pharmacology;
Tumor Cells, Cultured;
U937 Cells;
Young Adult
- From:
Journal of Experimental Hematology
2007;15(4):738-742
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to explore the correlation between apoptosis-related gene pnas-2 and leukemia. The RT-PCR was performed to detect the expression levels of pnas-2 gene in NB4, K562, U937 cells before and after treatment with AS(4)S(4), and to analysis the expression change of pnas-2 gene in bone marrow cells from patients with acute leukemia before and after chemotherapy. The results showed that the expression of pnas-2 gene in arsenic sulfide treated NB4 cells was down regulated in time-dependent manner, but the same outcome in K562 and U937 cells after being treated with AS(4)S(4) was not found. The positive expression rate of pnas-2 in cells from untreated patients with acute leukemia was 100%, and was significantly higher than that in normal control group. After chemotherapy, the expression was negative in complete remission patients, whereas in no-remission patients there were no significant differences of expression of pnas-2 before and after treatment. It is concluded that the pnas-2 gene may be closely related with apotosis of arsenic sulfide treated APL cells, and may consider as a molecular biological remission marker in acute leukemia.
