Effect of Shenluotong on secretion of ECM and expression of TGF-beta1 in rats mesangial cells of rats.
- Author:
Ya-li WANG
1
;
Yu-yong ZHAO
;
Zhi-qiang CHEN
;
Qing-you XU
;
Hua-zhou XU
;
Tong-zhong LI
;
Xian-ming HOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Astragalus membranaceus; chemistry; Cells, Cultured; Collagen Type IV; metabolism; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Extracellular Matrix; metabolism; Female; Fibronectins; metabolism; Glomerular Mesangium; cytology; Male; Materia Medica; isolation & purification; pharmacology; Oligochaeta; chemistry; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley; Salvia miltiorrhiza; chemistry; Transforming Growth Factor beta; biosynthesis; genetics; Transforming Growth Factor beta1
- From: China Journal of Chinese Materia Medica 2005;30(3):201-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Shenluotong on the expression of transforming growth factor-beta1 (TGF-beta1) and extracellular matrix (ECM) in Ang II-induced MCs.
METHODFibronectin (FN) and collagen type IV (Col IV) of extracellular matrix were detected by enzyme-linked immunosorbent assay; the expression of TGF-beta1 mRNA were measured by semi-quantitative reverse transcription-polymerase chain reaction.
RESULTA positive correlation between TGF-beta1 and ECM were found in the present study. FN, Col IV and TGF-beta1 mRNA were inhibited by Shenluotong significantly.
CONCLUSIONShenluotong can decrease the accumulation of ECM and inhibit the expression of TGF-beta1, suggesting further that shenluotong can be used to prevent and treat various glomerular diseases and delay glomerular sclerosis.
