Optimization of cell growth and 20-hydroxyecdysone production in cell suspension culture of Vitex glabrata R. Br.
- Author:
Duangjai SINLAPARAYA
1
;
Preeyada DUANGHAKLANG
;
Sanha PANICHAJAKUL
Author Information
1. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002 Thailand.
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
methods;
Culture Media;
Ecdysterone;
biosynthesis;
Vitex;
cytology;
metabolism
- From:
Chinese Journal of Biotechnology
2007;23(6):1033-1036
- CountryChina
- Language:Chinese
-
Abstract:
The effects of the cultivation media, plant growth regulators and inoculum size on the cell growth and 20-hydroxyecdysone production in suspension cultures of Vitex glabrata R. Br. were investigated. The cell growth and 20-hydroxyecdysone formation reach the highest when cells are cultured in the Gamborg's B5 medium supplemented with 2.0 mg/L BAP (6-benzylaminopurine) and 1.0 mg/L 2,4-D. The maximum 20-hydroxyecdysone productivity, of about 1.l mg/L/day, was observed in the culture with 20% PCV (packed cell volume) of inoculum size. These data also show that the increment of the inoculum size to 20% PCV could increase the productivity in 7-folds.
