Cloning and functional analysis of two regulators involved in regulation of cellulase formation in Trichoderma koningii.
- Author:
Min LING
1
;
Yongling QIN
;
Nan LI
;
Zhiqun LIANG
Author Information
1. Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
- Publication Type:Journal Article
- MeSH:
Binding Sites;
Cellulase;
biosynthesis;
Endo-1,4-beta Xylanases;
genetics;
metabolism;
Escherichia coli;
genetics;
metabolism;
Gene Expression Regulation, Enzymologic;
Gene Expression Regulation, Fungal;
Promoter Regions, Genetic;
Trans-Activators;
genetics;
metabolism;
Trichoderma;
enzymology;
genetics
- From:
Chinese Journal of Biotechnology
2008;24(10):1808-1812
- CountryChina
- Language:Chinese
-
Abstract:
ACEI and Xyr1 are two regulators that potentially involve in regulation of cellulases and xylanases formation in Trichoderma reesei, they compete for a binding site in the xyn1 (Xylanase1-encoding) gene promoter. To further investigate the mechanism for the transcriptional regulation of cellulases, DNA-binding domains of both ACEI and Xyr1 in T. Koningii were expressed from E. coli. They both showed bindings to the cbh1 promoter fragment (-304 bp to -18 bp) by electrophoresis mobility shift assays, suggesting ACEI and Xyr1 not only compete for binding to xyn1 promoter but also to cbh1 promoter.
