Pre-L-methionine feeding strategy for S-adenosyl-L-methionine fermentative production.
- Author:
Jiepeng WANG
1
;
Tianwei TAN
Author Information
1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
- Publication Type:Journal Article
- MeSH:
Bioreactors;
microbiology;
Fermentation;
Methionine;
analysis;
metabolism;
Mutation;
S-Adenosylmethionine;
biosynthesis;
Saccharomyces cerevisiae;
genetics;
growth & development;
metabolism
- From:
Chinese Journal of Biotechnology
2008;24(10):1824-1827
- CountryChina
- Language:Chinese
-
Abstract:
The yield of S-adenosyl-L-methionine (SAM) on high-cell-density fermentation by saccharomyces cerevisiae is mostly affected by the feeding strategy of pre-L-methionine. The mutant strain SAM0801 that could accumulate more SAM was used in this study. Six high-cell-density fermentation experiments in 5 L fermentor were investigated to get the optimal feeding time and amount of L-methionine. The results showed that when 40 g L-methionine was added in the fermentor after 30 h fermentation, a dry cell weight of 100 g/L was achieved. Under this condition, after 58 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 168 g/L and 14.48 g/L respectively.
